KATO III細(xì)胞，人胃癌細(xì)胞

簡要描述：KATO III細(xì)胞，人胃癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！

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KATO III細(xì)胞，人胃癌細(xì)胞

Organism	Homo sapiens, human
Tissue	stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac
Product Format	frozen
Morphology	spherical
Culture Properties	mixed, adherent and suspension
Biosafety Level	1
Disease	gastric carcinoma
Age	55 years adult
Gender	male
Ethnicity	Asian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data	55 years adult Asian male
Tumorigenic	Yes
Effects	Yes, in cheek pouches of anti thymocyte serum treated hamsters No, in nude mice

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. KATO III細(xì)胞，人胃癌細(xì)胞
Subculturing	Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C. *Subction Ratio:** A subction ratio of 1:2 to 1:8 is recommended Medium Renewal:* Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: 5% CO2 in air recommended Temperature: 37°C

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KATO III細(xì)胞，人胃癌細(xì)胞